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POLARstar OMEGA
Product Details
The POLARstar® Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Equipped with BMG LABTECH’s ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications
The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with six detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®. For cell-based applications, the optional Atmospheric Control Unit (ACU) makes O2 and CO2 gas control possible.
Features
- Two matched PMTs for Simultaneous Dual Emission detection
- Full-spectrum UV/Vis absorbance measurements in less than 1 second
- Measurement in up to 384-well microplate format
- Top and bottom reading
- Plate shaking and uniform incubation up to 45 °C or 65 °C
- Well-scanning and real-time kinetic monitoring
- High-precision injectors with simultaneous reagent injection and detection
- Includes multi-user control and MARS data analysis software
- ACU compatible for O2 and CO2 control
- Compatible with LVis Plate and Stacker
Technical Specifications
Detection Modes |
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Measurement Modes |
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Microplate Formats |
6 to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL) |
Microplate Carrier |
Robot compatible |
Light Sources |
High energy xenon flash lamp |
Detectors |
Two low noise photomultiplier tubes CCD spectrometer |
Wavelength Selection |
Optical Filters: Ex and Em wheels for 8 filters each UV/Vis Absorbance Spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well |
Optical Filters |
Excitation and emission filter wheels for 8 filters each |
Optical Path Guides |
Top: Liquid filled light guides Bottom: Fiber optics |
Spectral Range |
Filters 240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM Spectrometer 220 - 1000 nm for ABS |
Sensitivity* |
FI < 10 pM (< 0.2 fmol/well Fluorescein, 384sv, 20 μL) FP < 5 mP SD at 1 nM Sodium Fluorescein TRF < 30 amol/well Europium High-End TRF for Omega < 3 amol/well Europium LUM < 20 amol/well ATP AlphaScreen® with flash lamp < 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL) ABS with Spectrometer Full spectrum captured in < 1 s/well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD |
Read Times |
Flying mode (1 flash) 9 s (96), 16 s (384) |
Reagent Injection |
Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 500 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing |
Shaking |
Linear, orbital, and double-orbital with user-definable time and speed |
Purge Gas Vent |
System to inject an atmosphere or to pull a vacuum into the reader |
Incubation |
+4 °C above ambient up to 45 °C or 65 °C The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. |
Software |
Multi-user Reader Control and MARS Data Analysis Software included. FDA 21 CFR Part 11 compliant |
Dimensions |
Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg |
Optional Accessories |
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LVis Plate |
Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy Dimensions: Conforms to SBS standards for microplates. |
Atmospheric Control Unit (ACU) |
Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg |
Stacker |
Magazines for up to 50 plates - continuous loading feature |
THERMOstar |
Microplate Incubator and Shaker |
Optical Filters |
Excitation and emission filter wheels for 8 filters each |
* |
LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates |
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