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Scie-Plas Complete Electroblotting System

Product Details

The TV100YK-MODSYS complete modular system provides a fully integrated solution with one gel tank for 2-D electrophoresis and electroblotting, using 10 x 10cm gels.

FEATURES

  • Complete System
    TV100YK-MODSYS unit with GRM, cooled gel tank and quick-fit tubing, lid, 2 (10 x 10cm; W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners, 2 (10 x 10cm) notched glass plates, 1 x dummy plate, 2 x 1mm thick 12-sample combs, casting base and 2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm spacers, 4 x 1.5mm thick 1-D preparatory combs, 10 x blanking ports and 1 x gel extraction platform; plus EBGRM, 2 x electroblotting cassettes and 4 x fibre pads
  • Unit Dimensions (W x D x H): 28 x 15 x 18cm
  • Active Gel Dimensions (W x H): 8 x 8.5cm
  • Sample Capacity: 2 x 1 / 2 x 12
  • Recommended Buffer Volume (PAGE GRM):
    Inner Buffer Chamber: 90ml; Gel Tank: 1600ml
  • Recommended Buffer Volume (CAPGRM):
    Upper Buffer Chamber: 200ml; Gel Tank: 1600ml
  • Active Transfer Area / Electroblotting Cassette: 71cm2
  • Recommended Power Supplies:
    Consort EV215 or EV261     

The TV400YK-MODSYS complete modular system provides a fully integrated solution with one gel tank for 2-D electrophoresis and electroblotting, using 20.5 x 20cm gels.

FEATURES

  • Complete System
    TV400YK-MODSYS unit with GRM, cooled gel tank and quick-fit tubing, lid, 2 (20.5 x 20cm; W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners, 2 (20.5 x 20cm) notched glass plates, 1 x dummy plate, 2 x 1mm thick 24-sample combs, casting base and 2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm thick spacers, 4 x 1.5mm thick 1-D preparatory combs, 10 x blanking ports and 1 x gel extraction platform; plus EBGRM, 2 x electroblotting cassettes and 4 x fibre pads
  • Unit Dimensions (W x D x H): 38 x 15 x 27.5cm
  • Active Gel Dimensions (W x H): 16.5 x 17.5cm
  • Sample Capacity: 2 x 1 / 2 x 24
  • Recommended Buffer Volume (PAGE GRM):
    Inner Buffer Chamber: 700ml; Gel Tank: 4200ml
  • Recommended Buffer Volume (CAPGRM):
    Upper Buffer Chamber: 300ml; Gel Tank: 4200ml
  • Active Transfer Area / Electroblotting Cassette: 289cm2
  • Recommended Power Supplies:
    Consort EV215 or EV261     

Technical Specifications

    TV100YK-MODSYS TV400YK-MODSYS
Unit Dimensions (W x D x H)   28 x 15 x 18cm 38 x 15 x 27.5cm
Inner Tank Dimensions (W x D x H)   16.5 x 11 x 15cm 27 x 12.5 x 25cm
Plate Dimensions (W x H x T)   10 x 10 x 0.2cm 20.5 x 20 x 0.4cm
Standard Spacer Dimensions (W x H x T)   1 x 10 x 0.1cm 2 x 20 x 0.1cm
Active Gel Dimensions (W x H)   8 x 8.5cm 16.5 x 17.5cm
Sample Capacity   2 x 1 / 2 x 12 2 x 1 / 2 x 24

Recommended Buffer Volume

(PAGE GRM)

Inner Buffer Chamber 90ml 700ml
Gel Tank 1600ml 4200ml

Recommended Running Conditions for

SDS-PAGE

Voltage 100 - 150V (10 - 15V/cm) 200 - 300V (10 - 15V/cm)
Current 10 - 15mA 30 - 40mA
Time 1 - 1.75h 2 - 2.5h
Capillary Dimensions Inner Diameter 1mm 1mm
Outer Diameter 5mm 5mm
Length 80mm 170mm
Recommended Buffer Volume (CAPGRM) Upper Buffer Chamber 200ml 300ml
Gel Tank 1600ml 4200ml

Recommended Running

Conditionsfor IEF of Capillary Gels

  Pre-run Separation End of Run
Voltage 200V          400V          500V 200V           400V           500V
Time 0.5h              3h             0.5h 0.5h        6h to 18h          0.5h
Volt-hours 100Vh      1200Vh      250Vh

100Vh  2400 to 7200Vh   2400h

Distance Between Plate Electrodes

in EBGRM

  6cm 6cm

Electroblotting Cassette Dimensions

(W x L)

  11.5 x 10.5cm 20.5 x 20cm
Active Transfer Area   71cm2 289cm2

Recommended Running Conditions

for Electroblotting

Voltage 100V 200V
Current 75mA (0.6-0.8mA/cm2) 150mA (0.8-1.0mA/cm2)
Time 4 - 6h 6h to overnight
Snap-lock Connectors for Cooling Coil Inner Diameter 10mm 10mm
Outer Diameter 12mm 12mm
Quick-fit Tubing Inner Diameter 10mm 10mm
Outer Diameter 12mm 12mm

Casting Base Silicone Seal

Dimensions (W x L x H)

  1.5 x 11 x 0.8cm 1.7 x 22.5 x 0.8cm
Power Output Connectors (diameter)   Shrouded, 4mm Shrouded, 4mm
Recommended Power Supplies   Consort EV215 or EV261 Consort EV215 or EV261

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Buying Options

Product Code Description List Price Offer Price
TV100-EBK Complete Electroblotting System for four 10 x 10cm POA Enquire
TV400-EBK Complete Electroblotting System for four 20 x 20cm POA Enquire

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